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CYTOGENETICS LABORATORY

Karyotyping, also known as routine chromosome analysis, is a procedure that examines chromosomes in a sample of cells. The structure of each chromosome is analyzed for abnormalties as well the total number of chromosomes are noted. The chromosomes are then arranged in a karyogram which lists the 22 pairs of autosomes and 1 pair of sex chromosomes. Routine chromosome analysis is a useful methodology to detect common, recurrent chromosome abnormalities for hematological disorders.

FISH analysis is a technique that is used to detect and localize the presence or absence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that are often derived from fragments of DNA that were isolated, purified, and amplified for use in the Human Genome Project. The probes bind to only those parts of the chromosome with which they show a high degree of sequence complimentarity. The cells are then viewed under the microscope to locate the site where the probe is bound to the chromosome.

This type of analysis is useful in locating deletions or duplications that are too small to be seen via karyotyping and the cost of the assay is minimal compared to other methods of genetic testing. FISH is routinely used to confirm certain hematological disorders such as AML or CML and to monitor treatment. The probes used in this assay are chromosome region specific so the clinician must specify the probe and/or the disease/syndrome they suspect or have diagnosed in the patient.

FISH ANALYSIS FOR HEMATOLOGY/ONCOLOGY

FISH analysis can be performed for the following cancers:

Acute Myelogenous Leukemia (AML)

B-cell Acute Lymphatic Leukemia (ALL)

Chronic Lymphatic Leukemia (CLL)

Chronic Myelogenous Leukemia (CML)

Eosinophilia

Lymphomas

Myelodysplastic syndrome (MDS)

Myeloproliferative Neoplasms

Multiple Myeloma

T-cell Acute Lymphoblastic Leukemia (T-ALL)

Other Malignancies

Please refer to the Cytogenetics for Hematology/Oncology Disorders test order form to order testing and to review the specimen requirements.


 

 

Contact Information

OUHSC Genetics Laboratory 1122 NE 13th Street, Suite 1400, Oklahoma City, OK 73104

Phone (405)271-3589 Fax (405)271-7117 After hours phone (405)496-9514

   


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